Journal: Stem Cell Research & Therapy

Article Title: Compared to the amniotic membrane, Wharton’s jelly may be a more suitable source of mesenchymal stem cells for cardiovascular tissue engineering and clinical regeneration

doi: 10.1186/s13287-017-0501-x

Figure Lengend Snippet: Representative images of multi-lineage differentiation potential of isolated WJ-MSCs and AM-MSCs. WJ-MSCs and AM-MSCs were induced to differentiate toward adipogenic lineage and verified by Alizarin Red S staining (magnification, ×100), osteogenic lineage and verified by Oil Red O staining (magnification, ×200), and chondrogenic lineage and verified by Alcian Blue staining (magnification, ×200). Scale bar, 100 μm. AM-MSCs amniotic membrane mesenchymal stem cells, WJ-MSCs Wharton’s jelly mesenchymal stem cells

Article Snippet: Sections (4 μm) were deparaffinized in xylene and stained with Alcian Blue Staining Kit (ScienCell, Carlsbad, CA, USA) according to the user’s manual.

Techniques: Isolation, Staining, Membrane

Journal: Stem Cells International

Article Title: Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson's Disease: A New Biological Therapy for Parkinson's Disease

doi: 10.1155/2020/4636397

Figure Lengend Snippet: Phenotypic characterization and differentiation of hUC-MSCs. (a) Flow cytometry showing that isolated cultured hUC-MSCs highly expressed CD105, CD73, and CD90 and were negative for hematopoietic markers CD19, CD34, and CD45 and for the lymphocyte surface marker HLA-DR. (b) (A) Gross morphology of hUC-MSCs under light microscopy, (B) oil-red-O staining for the adipogenic differentiation of hUC-MSCs after 3 weeks, (C) endogenous alkaline phosphatase staining for the osteogenic differentiation of hUC-MSCs, and (D) Alcian blue staining of hUC-MSCs after 3 weeks for chondrogenic differentiation (scale bar = 100 μ m).

Article Snippet: The sections were analyzed for chondrogenic differentiation using a commercially available Alcian blue kit (IHC World, Woodstock).

Techniques: Flow Cytometry, Isolation, Cell Culture, Marker, Light Microscopy, Staining

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways

doi: 10.1016/j.mcpro.2025.100962

Figure Lengend Snippet: Histological staining of extracellular matrix proteins in CAPJ-treated wound tissues. Rat skin cutaneous wound was treated with CAPJ for 60s. CAPJ-treated wound tissue section (CAPJ), with parallel untreated control (Ctrl), were prepared on day-3, -7, and -14, and subjected for histochemical staining by using Masson’s Trichrome Stain kit for collagen staining ( top panel, in dark blue color), Elastic Stain kit for elastin staining ( middle panel, in black color), and Alcian Blue Stain kit for hyaluronic acid (HA) staining ( button panel, in light-blue color). The red arrows were the extensively stained area for collagen and elastic fibers formation as well as the conjugates of HA and proteoglycans. Right panel correspond to high-magnification images of each cropped area on low-magnification image. Scale bars in low- and high-magnification images are 20 μm and 5 μm, respectively. CAPJ, cold atmospheric plasma jet.

Article Snippet: Tissue section (5 μm thick) was prepared in a standard fashion and observed in detail using Masson’s Trichrome Stain kit (StatLab, #KTMTR), Elastic Stain Kit (Modified Verhoeff's, ScyTek, ETS-1-IFU) and Alcian Blue (pH 2.5) Stain Kit (ScyTek, AFR-1-IFU).

Techniques: Staining, Control, Clinical Proteomics